CUTANA™ CUT&RUN assays

pAG-MNase is now available!

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CUTANA™ assays vastly outperform ChIP-Seq

When compared with ChIP-seq, CUTANA™ assays have lower sequencing requirements, improved Signal-to-Noise, and reduced assay time.

How do CUTANA™ CUT&RUN assays work?
CUTANA™ assays use a proprietary immunotethering approach similar to Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods. This approach can be used to map histone post-translational modifications (PTMs) and chromatin interactors with high sensitivity and resolution.

This approach leverages a factor specific antibody to tether a fusion of protein A, protein G and micrococcal nuclease (pAG-MNase) to genomic binding sites in intact cells, which is then activated by the addition of calcium to cleave DNA. The pAG-MNase enzyme is the first reagent in the CUTANA™ product line and is available now.

Overview of the approach
Cells (or nuclei) are immobilized on lectin-coated magnetic beads, permeabilized, and incubated with an antibody to a chromatin target (e.g. histone PTM or chromatin / DNA binding protein). Next, pAG-MNase is added and activated via Ca2+. The clipped chromatin fragments diffuse out, followed by DNA purification and next-generation sequencing. CUTANA™ assays vastly outperform those using chromatin immunoprecipitation, the current gold-standard genomic mapping assay.

CUTANA™ assay workflow

From cells to data in less than 4 days.

DAY 1-2

DNA purification

DAY 2-3

Library presentation for NGS

DAY 3

Sequencing

DAY 4

Data Analysis

CUTANA™ assays save 5-10x in sequencing requirements

CUTANA™ assays generate superior quality data compared to ChIP-seq using as little as 3 million sequencing reads.

Comparing data generated from standard ChIP-seq methods and sequencing depth (black) to CUTANA (blue). H3K27me3 and H3K4me3 ChIP-Seq data was sourced from ENCODE. Negative control data (yellow) was generated using a Rabbit IgG antibody in CUTANA. Each dataset of CUTANA was acquired using 0.5 million cells.

CUTANA™ assays for ChIC / CUT&RUN

Low background. Highly reproducible. Cut through the noise.

A representative 350 kb region of an H3K4me1 profile in K-562 cells, generated using CUTANA (yellow panels), native ChIP-seq (blue panels), or cross-linked ChIP-seq (green panels). All data were generated by EpiCypher and are expressed as reads per million (RPM). Color- coded gradient (to left) represents signal/noise (S/N) ratios determined by genome-wide analysis (bamFingerprint data, not shown).

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CUTANA™ pAG-MNase for ChIC/CUT&RUN 

Recombinantly produced in E. coli, CUTANA™ pAG-MNase for ChIC/CUT&RUN Workflows is a fusion of Proteins A and G to Micrococcal Nuclease. This construct is useful in performing Chromatin Immunocleavage (ChIC)1 and Cleavage Under Targets and Release Using Nuclease (CUT&RUN)2,3. CUTANA™ pAG-MNase contains a C-terminal 6xHis epitope tag.

Technical Data Sheet
CUTANA™ CUT&RUN Protocol for Histone PTMs

50 Rxns:                 $300
250 Rxns:               $1,250
>2500 Rxns:           ** Contact for pricing **

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About EpiCypher

A pioneer in the field of epigenetics and chromatin biology, EpiCypher is a biotechnology company developing transformative technologies to advance chromatin science and improve human health.

EpiCypher manufactures and sells a series of products and assay platforms that use recombinant “designer” nucleosomes (dNucs), including SNAP-ChIP® for quantitative ChIP applications, EpiDyne® for nucleosome remodeling assays, dCypher® to interrogate epigenetic regulators, recombinant histone binding proteins and enzymes, peptides and antibodies; and a broad range of custom substrate / assay development services.

General inquiries: info@epicypher.com


Questions? Give us a call.
(855) 374-2461