CUTANA™ ChIC/CUT&RUN assays

pAG-MNase & positive control antibody are now available!

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CUTANA™ assays vastly outperform ChIP-Seq

When compared with ChIP-seq, CUTANA™ assays have lower sequencing requirements, improved signal-to-noise, and reduced assay time.

How do CUTANA™ assays work?
CUTANA™ assays use a proprietary immunotethering approach similar to Chromatin ImmunoCleavage (ChIC) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) methods. This approach can be used to map histone post-translational modifications (PTMs) and chromatin interactors (e.g. transcription factors) with high sensitivity and resolution.

Cells (or nuclei) are immobilized on lectin-coated magnetic beads, permeabilized, and incubated with an antibody to a chromatin target (e.g. histone PTM or chromatin / DNA binding protein). Next, a fusion of protein A, protein G, and micrococcal nuclease (pAG-MNase) is added, which binds to the antibody, and is then activated via Ca2+ to cleave the DNA. The clipped chromatin fragments can then be easily purified and analyzed by next-generation sequencing.

CUTANA™ assays have clear advantages over ChIP-seq, the current gold-standard genomic mapping assay.

CUTANA™ assay workflow

From cells to data in less than 4 days.

DAY 1-2

DNA Purification

DAY 2-3

Library Preparation for NGS

DAY 3

Sequencing

DAY 4

Data Analysis

CUTANA™ CUT&RUN Protocol
EpiCypher's team of scientists have extensively optimized our CUT&RUN protocol to ensure consistent yields, high reproducibility, and high signal-to-noise across a variety of chromatin binding targets:
  • Histone PTMs
  • Transcription Factors
  • Chromatin Regulators and Remodelers
In addition, we have addressed common issues (e.g. bead clumping/loss), and enabled multi-channel high-throughput sample handling via an 8-strip tube format. The protocol includes notes throughout, critical steps, pictures for clarity, and a FAQ section.

Generate high quality data using as few as 3 million reads

Low background. Highly reproducible. Cut through the noise.

A representative 350 kb region of an H3K4me1 profile in K-562 cells, generated using CUTANA (yellow panels), native ChIP-seq (blue panels), or cross-linked ChIP-seq (green panels). All data were generated by EpiCypher and are expressed as reads per million (RPM). Color- coded gradient (to right) represents signal/noise (S/N) ratios determined by genome-wide analysis (bamFingerprint data, not shown).

CUTANA™ Supporting Reagents and Tools
Available To Order Now

H3K4me3 CUT&RUN Compatible Positive Control Antibody
 

The first ever antibody with demonstrated, reliable performance in CUT&RUN assays. Delivers genome-wide enrichment highly correlated with H3K4me3 ChIP-seq data.

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CUTANA™ pAG-MNase for ChIC / CUT&RUN Assays
 

Fusion of proteins A and G to Micrococcal Nuclease containing a C-terminal 6xHis epitope tag. For more information, see the technical data sheet. Available in 50 or 250 reaction pack sizes.

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CUTANA™ Rabbit IgG CUT&RUN Negative Control Antibody
 

Examine non-specific background signal in CUT&RUN experiments using this rabbit IgG antibody. Also compatible with other applications (e.g. ChIP-seq).

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Magnetic Separation
Rack


Magnetic Rack designed to hold 16 0.2ml PCR tubes. Two possible tube positions for large and small volumes. Accommodates multichannel pipette for easy sample handling and increased throughput.

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Concanavalin A (ConA) Coated Magnetic Beads

Concanavalin A is a lectin that binds to extracellular glycoproteins. ConA magnetic beads can be used to immobilize cells or nuclei for CUT&RUN applications.

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CUTANA™ CUT&RUN 8-strip 0.2mL tubes
 

PCR 8-well strip tubes with individual attached flat caps enable increased throughput and improved sample handling for CUT&RUN experiments. DNase and RNase free. 

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About EpiCypher

A pioneer in the field of epigenetics and chromatin biology, EpiCypher is a biotechnology company developing transformative technologies to advance chromatin science and improve human health.

EpiCypher manufactures and sells a series of products and assay platforms that use recombinant “designer” nucleosomes (dNucs), including SNAP-ChIP® for quantitative ChIP applications, EpiDyne® for nucleosome remodeling assays, dCypher® to interrogate epigenetic regulators, recombinant histone binding proteins and enzymes, peptides and antibodies; and a broad range of custom substrate / assay development services.

General inquiries: info@epicypher.com


Questions? Give us a call.
(855) 374-2461