EpiDyne® Assays

Chromatin Remodeling Studies Made Easy

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Chromatin remodeling enzymes are high value therapeutic targets that require nucleosome substrates.

ATP-dependent chromatin remodeling enzymes are a rapidly emerging therapeutic target. Components of SWI/SNF, the most commonly studied chromatin remodeling complex, are mutated in ~20% of all human cancers. Traditional assays used to identify chromatin remodeling inhibitors indirectly measure ATP consumption. This makes them prone to identification of nonspecific inhibitors of ATP-dependent enzymes. Further, these assays do not directly monitor chromatin remodeling activity. Therefore, there is a need in the field for defined nucleosome substrates that can be used to directly quantify chromatin remodeling activity.

Subunit Cancer
ARID1A Ovarian, hepatocellular, bladder, gastric, endometrioid, pancreatic, colon, lung, neuroblastoma, Burkitt lymphoma
ARID1B Melanoma, neuroblastoma, hepatocellular, pancreatic, liver
PBRM1 Renal cell carcinoma, breast, gastic, pancreatic
SMARCA2 Lung, colon, breast
SMARCA4 Lung medulloblastoma, Burkitt lymphoma, SCCOHT
SMARCB1 Rhabdoid tumor, familial schwannomatosis
SMARCE1 Spinal meningioma
BRD7 Breast

Adapted from Heming et al.

EpiDyne® assays use recombinant nucleosome substrates and are compatible with high-throughput screening (HTS) readouts

The EpiDyne® Collection

Recombinant nucleosome-based assays for functional chromatin remodeling studies

EpiDyne-FRET

In EpiDyne-FRET, the addition of a chromatin remodeling enzyme repositions the histone octamer, which increases the distance between Cy3 and Cy5 and leads to a reduction in FRET signal.
  • Directly monitors remodeling activity
  • HTS compatible protocol: one-step, no-wash
  • Easy to use - quantitative readout
  • Useful for inhibitor screening
  • Highly tractable assay, adaptable to different classes of remodelers

Restriction Enzyme Accessibility (REA)

In EpiDyne-REA, a terminally positioned histone octamer shields a restriction enzyme recognition site (DpnII GATC), which is exposed by ATP-dependent nucleosome remodeling activity.
  • Highly accurate, flexible, and reliable monitoring of remodeling activity
  • Measures directional shifting
  • Useful for mechanistic studies
  • Multiple readouts possible including HTS-compatible radiometric assays using a GATC-targeting methyltransferase. Inquire!
Chromatin Remodeling Enzymes
To complement our EpiDyne substrates, EpiCypher offers a range of remodeling enzymes, including SMARCA2 and SMARCA4 (the ATPases of the SWI/SNF complex) and the ACF ISWI-type complex. Each protein has passed our rigorous quality control metrics and is validated in EpiDyne-FRET.
EpiDyne® Custom Reagents & Drug Discovery Services
EpiCypher's FRET assay using SMARCA4 enzyme (Catalog No. 15-1014) and EpiDyne-FRET nucleosome remodeling substrate (Catalog No. 16-4201) was optimized in 384 well plates for high-throughput screening applications.
EpiCypher offers customized services including:
  • Custom remodeling substrates
  • HTS assay optimization
  • Inhibitor screening
  • Specificity testing
These services are available in multiple assay formats:
  • FRET / TR-FRET
  • Radioactivity (DAM methyltransferase)
  • AlphaScreen / AlphaLISA
  • Other


About EpiCypher

A pioneer in the field of epigenetics and chromatin biology, EpiCypher is a biotechnology company developing transformative technologies to advance chromatin science and improve human health.

EpiCypher manufactures and sells a series of products and assay platforms that use recombinant “designer” nucleosomes (dNucs), including SNAP-ChIP® for quantitative ChIP applications, EpiDyne® for nucleosome remodeling assays, dCypher® to interrogate epigenetic regulators, recombinant histone binding proteins and enzymes, peptides and antibodies; and a broad range of custom substrate / assay development services.

General inquiries: info@epicypher.com


Questions? Give us a call.
(855) 374-2461