Sample Normalization and Antibody Profiling for ChIP (SNAP-ChIP) Spike-In Controls provide a powerful new tool to generate highly reliable ChIP-seq data.
This technology uses a pool of recombinant nucleosomes carrying widely studied histone post-translational modifications (PTMs), each denoted by unique DNA barcodes.
SNAP-ChIP Spike-Ins are compatible with both native and cross-linked workflows. Simply spike-in the panel of interest prior to IP.
The nucleosomal DNA barcodes on the spike-ins can then be detected by qPCR or NGS and used to quantify antibody performance and normalize samples for reliable cross-experimental comparisons (Grzybowski et al. Nat Protoc 2019).